Review




Structured Review

10X Genomics visium spatial gene expression slides
This figure summarizes the machine learning-guided workflow used to define tumor, boundary, and stromal domains in hepatocellular carcinoma specimens. ( a) Study workflow. Eleven treatment-naive HCC resection specimens, including seven HBV-related and four non-B non-C cases, were profiled <t>using</t> <t>10x</t> <t>Visium</t> spatial transcriptomics. CancerFinder was used to estimate spot-level malignancy probability, SpaceFlow was used for spatially regularized clustering, and the resulting outputs were integrated with histologic review to define three spatial domains: Tumor, Boundary, and Stroma. A signed distance-to-border axis was constructed for continuous spatial gradient analyses. ( b) Hematoxylin and eosin-stained sections from representative HBV-related and NBNC specimens. ( c) CancerFinder-derived cancer/normal classification maps for the same specimens. Blue indicates normal or low-malignancy-probability regions, and orange indicates cancer or high-malignancy-probability regions. ( d) Integrated three-domain spatial annotation maps. Dark red indicates Boundary, cyan indicates Stroma, and dark blue indicates Tumor. ( e) Boxplots of estimated malignant cell proportion across ordered spatial subdomains in the representative specimens. The red line indicates mean distance from the tumor for each subdomain, supporting concordance between the inferred malignancy gradient and the spatial domain hierarchy.
Visium Spatial Gene Expression Slides, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/visium+spatial+gene+expression+slides/bio_rxiv__64898__2026__06__02__729569-152-10-15
Average 86 stars, based on 1 article reviews
visium spatial gene expression slides - by Bioz Stars, 2026-09
86/100 stars

Images

1) Product Images from "Spatial Transcriptomics Reveals a Conserved Border Niche and Etiology-Associated Immune Rewiring in Hepatocellular Carcinoma"

Article Title: Spatial Transcriptomics Reveals a Conserved Border Niche and Etiology-Associated Immune Rewiring in Hepatocellular Carcinoma

Journal: bioRxiv

doi: 10.64898/2026.06.02.729569

This figure summarizes the machine learning-guided workflow used to define tumor, boundary, and stromal domains in hepatocellular carcinoma specimens. ( a) Study workflow. Eleven treatment-naive HCC resection specimens, including seven HBV-related and four non-B non-C cases, were profiled using 10x Visium spatial transcriptomics. CancerFinder was used to estimate spot-level malignancy probability, SpaceFlow was used for spatially regularized clustering, and the resulting outputs were integrated with histologic review to define three spatial domains: Tumor, Boundary, and Stroma. A signed distance-to-border axis was constructed for continuous spatial gradient analyses. ( b) Hematoxylin and eosin-stained sections from representative HBV-related and NBNC specimens. ( c) CancerFinder-derived cancer/normal classification maps for the same specimens. Blue indicates normal or low-malignancy-probability regions, and orange indicates cancer or high-malignancy-probability regions. ( d) Integrated three-domain spatial annotation maps. Dark red indicates Boundary, cyan indicates Stroma, and dark blue indicates Tumor. ( e) Boxplots of estimated malignant cell proportion across ordered spatial subdomains in the representative specimens. The red line indicates mean distance from the tumor for each subdomain, supporting concordance between the inferred malignancy gradient and the spatial domain hierarchy.
Figure Legend Snippet: This figure summarizes the machine learning-guided workflow used to define tumor, boundary, and stromal domains in hepatocellular carcinoma specimens. ( a) Study workflow. Eleven treatment-naive HCC resection specimens, including seven HBV-related and four non-B non-C cases, were profiled using 10x Visium spatial transcriptomics. CancerFinder was used to estimate spot-level malignancy probability, SpaceFlow was used for spatially regularized clustering, and the resulting outputs were integrated with histologic review to define three spatial domains: Tumor, Boundary, and Stroma. A signed distance-to-border axis was constructed for continuous spatial gradient analyses. ( b) Hematoxylin and eosin-stained sections from representative HBV-related and NBNC specimens. ( c) CancerFinder-derived cancer/normal classification maps for the same specimens. Blue indicates normal or low-malignancy-probability regions, and orange indicates cancer or high-malignancy-probability regions. ( d) Integrated three-domain spatial annotation maps. Dark red indicates Boundary, cyan indicates Stroma, and dark blue indicates Tumor. ( e) Boxplots of estimated malignant cell proportion across ordered spatial subdomains in the representative specimens. The red line indicates mean distance from the tumor for each subdomain, supporting concordance between the inferred malignancy gradient and the spatial domain hierarchy.

Techniques Used: Spatial Transcriptomics, Construct, Staining, Derivative Assay

Related Articles

Gene Expression:

Article Title: Evolution of songbird vocal imitation involved multiple cellular innovations
Article Snippet: .. Sections were carefully mounted onto Visium Spatial Gene Expression Slides (10x Genomics), ensuring placement within the capture area, and immediately returned to −80 °C for short-term storage or processed directly. ..

Article Title: Spatial Transcriptomics Reveals a Conserved Border Niche and Etiology-Associated Immune Rewiring in Hepatocellular Carcinoma
Article Snippet: .. Specimens were OCT-embedded, snap-frozen, and cryosectioned at 10 um onto Visium Spatial Gene Expression slides (10x Genomics). .. Spatially barcoded gene expression profiling was performed using the 10x Visium platform, with libraries sequenced on an Illumina NovaSeq system.

Article Title: Non-invasive radiogenomic mapping of the SMARCAL1-driven ferroptotic niche is associated with longitudinal MRD-negative surveillance in early-stage NSCLC
Article Snippet: .. Fresh-frozen tumor tissues preserved in OCT compound were cryosectioned at 10 μm and mounted on 10x Genomics Visium Spatial Gene Expression slides (4 capture areas × 4,992 barcoded 55 μm spots, 100 μm center-to-center). .. Sections underwent H&E staining and ultra-high-resolution imaging on a Leica Aperio AT2 scanner.

Article Title: E-cadherin inactivation shapes tumor microenvironment specificities in invasive lobular breast cancer.
Article Snippet: .. Cryostat sections of 10 μm thickness were placed on Visium Spatial Gene Expression slides (10X Genomics, PN-1000184). ..

Article Title: Multiomic Analysis Reveals an IFN-driven Cellular Landscape Effectively Targeted by Ruxolitinib in Hailey-Hailey Disease
Article Snippet: .. Samples were sectioned at 12 μm and mounted onto Visium Spatial Gene Expression slides (10x Genomics). ..

Article Title: Cross-tissue atlas of mucosa-associated lymphoid tissue lymphomas reveals intratumoral heterogeneity and microenvironmental subtypes
Article Snippet: .. Tissue sections of 5 μm thickness were mounted onto Visium Spatial Gene Expression Slides (10x Genomics). .. H&E staining was performed using Mayer’s hematoxylin (Millipore Sigma), bluing reagent (Dako, Agilent), and alcoholic eosin (Millipore Sigma).

Article Title: A phenotype-to-mechanism framework links phenome-wide comorbidity architecture to molecular mechanisms and therapeutic discovery in complex diseases
Article Snippet: .. Formalin fixed paraffin embedded specimens from HS lesional samples were sectioned onto Visium Spatial Gene Expression Slides (10X Genomics) by the Experimental Pathology Core at NYU Langone (IRB number: s19-00841). ..

Article Title: Uncovering the signatures of aging and senescence in the human dorsolateral prefrontal cortex
Article Snippet: Visium spatially resolved gene expression data was generated according to the Visium Spatial Gene Expression User Guide (10x Genomics, CG000239 Rev F). .. Briefly, tissue sections collected onto Visium Spatial Gene Expression Slides (10x Genomics; 1000185) were fixed in chilled methanol and an abbreviated hematoxylin and eosin protocol was used for histological staining. .. Visium spatially resolved gene expression data was generated according to the Visium Spatial Gene Expression User Guide (10x Genomics, CG000239 Rev F).

Formalin-fixed Paraffin-Embedded:

Article Title: A phenotype-to-mechanism framework links phenome-wide comorbidity architecture to molecular mechanisms and therapeutic discovery in complex diseases
Article Snippet: .. Formalin fixed paraffin embedded specimens from HS lesional samples were sectioned onto Visium Spatial Gene Expression Slides (10X Genomics) by the Experimental Pathology Core at NYU Langone (IRB number: s19-00841). ..

Staining:

Article Title: Uncovering the signatures of aging and senescence in the human dorsolateral prefrontal cortex
Article Snippet: Visium spatially resolved gene expression data was generated according to the Visium Spatial Gene Expression User Guide (10x Genomics, CG000239 Rev F). .. Briefly, tissue sections collected onto Visium Spatial Gene Expression Slides (10x Genomics; 1000185) were fixed in chilled methanol and an abbreviated hematoxylin and eosin protocol was used for histological staining. .. Visium spatially resolved gene expression data was generated according to the Visium Spatial Gene Expression User Guide (10x Genomics, CG000239 Rev F).



Similar Products

86
10X Genomics visium spatial gene expression slides
This figure summarizes the machine learning-guided workflow used to define tumor, boundary, and stromal domains in hepatocellular carcinoma specimens. ( a) Study workflow. Eleven treatment-naive HCC resection specimens, including seven HBV-related and four non-B non-C cases, were profiled <t>using</t> <t>10x</t> <t>Visium</t> spatial transcriptomics. CancerFinder was used to estimate spot-level malignancy probability, SpaceFlow was used for spatially regularized clustering, and the resulting outputs were integrated with histologic review to define three spatial domains: Tumor, Boundary, and Stroma. A signed distance-to-border axis was constructed for continuous spatial gradient analyses. ( b) Hematoxylin and eosin-stained sections from representative HBV-related and NBNC specimens. ( c) CancerFinder-derived cancer/normal classification maps for the same specimens. Blue indicates normal or low-malignancy-probability regions, and orange indicates cancer or high-malignancy-probability regions. ( d) Integrated three-domain spatial annotation maps. Dark red indicates Boundary, cyan indicates Stroma, and dark blue indicates Tumor. ( e) Boxplots of estimated malignant cell proportion across ordered spatial subdomains in the representative specimens. The red line indicates mean distance from the tumor for each subdomain, supporting concordance between the inferred malignancy gradient and the spatial domain hierarchy.
Visium Spatial Gene Expression Slides, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/visium+spatial+gene+expression+slides/bio_rxiv__64898__2026__06__02__729569-152-10-15
Average 86 stars, based on 1 article reviews
visium spatial gene expression slides - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
10X Genomics visium spatial gene expression slide
This figure summarizes the machine learning-guided workflow used to define tumor, boundary, and stromal domains in hepatocellular carcinoma specimens. ( a) Study workflow. Eleven treatment-naive HCC resection specimens, including seven HBV-related and four non-B non-C cases, were profiled <t>using</t> <t>10x</t> <t>Visium</t> spatial transcriptomics. CancerFinder was used to estimate spot-level malignancy probability, SpaceFlow was used for spatially regularized clustering, and the resulting outputs were integrated with histologic review to define three spatial domains: Tumor, Boundary, and Stroma. A signed distance-to-border axis was constructed for continuous spatial gradient analyses. ( b) Hematoxylin and eosin-stained sections from representative HBV-related and NBNC specimens. ( c) CancerFinder-derived cancer/normal classification maps for the same specimens. Blue indicates normal or low-malignancy-probability regions, and orange indicates cancer or high-malignancy-probability regions. ( d) Integrated three-domain spatial annotation maps. Dark red indicates Boundary, cyan indicates Stroma, and dark blue indicates Tumor. ( e) Boxplots of estimated malignant cell proportion across ordered spatial subdomains in the representative specimens. The red line indicates mean distance from the tumor for each subdomain, supporting concordance between the inferred malignancy gradient and the spatial domain hierarchy.
Visium Spatial Gene Expression Slide, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/visium+spatial+gene+expression+slides/pm42248577-186-37-42
Average 86 stars, based on 1 article reviews
visium spatial gene expression slide - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
10X Genomics visium spatial gene expression slide kit
This figure summarizes the machine learning-guided workflow used to define tumor, boundary, and stromal domains in hepatocellular carcinoma specimens. ( a) Study workflow. Eleven treatment-naive HCC resection specimens, including seven HBV-related and four non-B non-C cases, were profiled <t>using</t> <t>10x</t> <t>Visium</t> spatial transcriptomics. CancerFinder was used to estimate spot-level malignancy probability, SpaceFlow was used for spatially regularized clustering, and the resulting outputs were integrated with histologic review to define three spatial domains: Tumor, Boundary, and Stroma. A signed distance-to-border axis was constructed for continuous spatial gradient analyses. ( b) Hematoxylin and eosin-stained sections from representative HBV-related and NBNC specimens. ( c) CancerFinder-derived cancer/normal classification maps for the same specimens. Blue indicates normal or low-malignancy-probability regions, and orange indicates cancer or high-malignancy-probability regions. ( d) Integrated three-domain spatial annotation maps. Dark red indicates Boundary, cyan indicates Stroma, and dark blue indicates Tumor. ( e) Boxplots of estimated malignant cell proportion across ordered spatial subdomains in the representative specimens. The red line indicates mean distance from the tumor for each subdomain, supporting concordance between the inferred malignancy gradient and the spatial domain hierarchy.
Visium Spatial Gene Expression Slide Kit, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/visium+spatial+gene+expression+slides/bio_rxiv__64898__2026__06__01__729268-219-25-31
Average 86 stars, based on 1 article reviews
visium spatial gene expression slide kit - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
10X Genomics visium spatial gene expression slide reagent kit
This figure summarizes the machine learning-guided workflow used to define tumor, boundary, and stromal domains in hepatocellular carcinoma specimens. ( a) Study workflow. Eleven treatment-naive HCC resection specimens, including seven HBV-related and four non-B non-C cases, were profiled <t>using</t> <t>10x</t> <t>Visium</t> spatial transcriptomics. CancerFinder was used to estimate spot-level malignancy probability, SpaceFlow was used for spatially regularized clustering, and the resulting outputs were integrated with histologic review to define three spatial domains: Tumor, Boundary, and Stroma. A signed distance-to-border axis was constructed for continuous spatial gradient analyses. ( b) Hematoxylin and eosin-stained sections from representative HBV-related and NBNC specimens. ( c) CancerFinder-derived cancer/normal classification maps for the same specimens. Blue indicates normal or low-malignancy-probability regions, and orange indicates cancer or high-malignancy-probability regions. ( d) Integrated three-domain spatial annotation maps. Dark red indicates Boundary, cyan indicates Stroma, and dark blue indicates Tumor. ( e) Boxplots of estimated malignant cell proportion across ordered spatial subdomains in the representative specimens. The red line indicates mean distance from the tumor for each subdomain, supporting concordance between the inferred malignancy gradient and the spatial domain hierarchy.
Visium Spatial Gene Expression Slide Reagent Kit, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/visium+spatial+gene+expression+slides/expression+gene+slides+spatial+visium/pmc13176823-47-1-9
Average 86 stars, based on 1 article reviews
visium spatial gene expression slide reagent kit - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

Image Search Results


This figure summarizes the machine learning-guided workflow used to define tumor, boundary, and stromal domains in hepatocellular carcinoma specimens. ( a) Study workflow. Eleven treatment-naive HCC resection specimens, including seven HBV-related and four non-B non-C cases, were profiled using 10x Visium spatial transcriptomics. CancerFinder was used to estimate spot-level malignancy probability, SpaceFlow was used for spatially regularized clustering, and the resulting outputs were integrated with histologic review to define three spatial domains: Tumor, Boundary, and Stroma. A signed distance-to-border axis was constructed for continuous spatial gradient analyses. ( b) Hematoxylin and eosin-stained sections from representative HBV-related and NBNC specimens. ( c) CancerFinder-derived cancer/normal classification maps for the same specimens. Blue indicates normal or low-malignancy-probability regions, and orange indicates cancer or high-malignancy-probability regions. ( d) Integrated three-domain spatial annotation maps. Dark red indicates Boundary, cyan indicates Stroma, and dark blue indicates Tumor. ( e) Boxplots of estimated malignant cell proportion across ordered spatial subdomains in the representative specimens. The red line indicates mean distance from the tumor for each subdomain, supporting concordance between the inferred malignancy gradient and the spatial domain hierarchy.

Journal: bioRxiv

Article Title: Spatial Transcriptomics Reveals a Conserved Border Niche and Etiology-Associated Immune Rewiring in Hepatocellular Carcinoma

doi: 10.64898/2026.06.02.729569

Figure Lengend Snippet: This figure summarizes the machine learning-guided workflow used to define tumor, boundary, and stromal domains in hepatocellular carcinoma specimens. ( a) Study workflow. Eleven treatment-naive HCC resection specimens, including seven HBV-related and four non-B non-C cases, were profiled using 10x Visium spatial transcriptomics. CancerFinder was used to estimate spot-level malignancy probability, SpaceFlow was used for spatially regularized clustering, and the resulting outputs were integrated with histologic review to define three spatial domains: Tumor, Boundary, and Stroma. A signed distance-to-border axis was constructed for continuous spatial gradient analyses. ( b) Hematoxylin and eosin-stained sections from representative HBV-related and NBNC specimens. ( c) CancerFinder-derived cancer/normal classification maps for the same specimens. Blue indicates normal or low-malignancy-probability regions, and orange indicates cancer or high-malignancy-probability regions. ( d) Integrated three-domain spatial annotation maps. Dark red indicates Boundary, cyan indicates Stroma, and dark blue indicates Tumor. ( e) Boxplots of estimated malignant cell proportion across ordered spatial subdomains in the representative specimens. The red line indicates mean distance from the tumor for each subdomain, supporting concordance between the inferred malignancy gradient and the spatial domain hierarchy.

Article Snippet: Specimens were OCT-embedded, snap-frozen, and cryosectioned at 10 um onto Visium Spatial Gene Expression slides (10x Genomics).

Techniques: Spatial Transcriptomics, Construct, Staining, Derivative Assay